Specimen should be transported as soon as possible. When transportation is delayed, leave at room temperature.
The assay will be reported as positive, negative, or equivocal. For positive specimens, the ratio of the specimen`s result to the positive cut off will be reported (>1). Equivocal results are values that fall between 75% and 100% of the positive cut off value (ratio = 0.75-1). If an equivocal test result is obtained, another blood specimen should be submitted, if CMV infection is still suspected.
LIMITATIONS OF TEST:
Studies have shown that the CMV DNA quantitative assay correlates well with CMV antigenemia in immunocompromised patients such as those with HIV infection or those who have received a solid organ or bone marrow transplant. However, the ratio of CMV DNA predictive of CMV disease (versus infection) in each of these patient populations has not been determined with certainty. Furthermore, a negative result does not exclude the possibility of CMV infection since very low levels of infection or sampling error may cause a false negative result or the disease may be restricted to a specific body organ.