Specimen should be transported as soon as possible. When transportation is delayed, leave at room temperature.
No cryptococcal antigen detected
LIMITATIONS OF TEST:
False negative results may occur due to a low concentration of the polysaccharide antigen or due to a prozone effect at high antigen concentrations. False positive results may occur due to invasive infections with the yeast Trichosporon beigelii,Capnocytophaga species, or Rothia(Stomatococcus)mucilaginosus.
A titer will be reported for all positive results. A higher titer usually corresponds with worse disease. Pretreatment of blood specimens with pronase reduces false-positives and increases the sensitivity of the method. A fungal culture should also be performed for patients who are suspected of having cryptococcosis. Antigen detection may precede the detection of viable organisms in cerebrospinal fluid Gram stains and fungal culture. The India Ink preparation has been replaced by the cryptococcal antigen procedure due to the increased sensitivity. A cryptococcal antigen will routinely be performed on all cerebrospinal fluid specimens submitted for a fungal culture providing an adequate volume of specimen is submitted.