Excessive hemolysis, gross contamination of the specimen, chylous serum, improper specimen storage, inappropriate specimen container, insufficient specimen volume.
7 mL blood (1 mL serum)
Specimen should be transported as soon as possible. When transportation is delayed, refrigerate.
The screen detects antibody produced against all chlamydial species. The determination of species or type specific antibodies to C. psittaci, C. trachomatis, and the C. pneumoniae is complicated by cross reactive antibody or non-specific stimulation of antichlamydial antibodies. Thus, a panel of Chlamydia must be tested to determine specific titers and establish the strongest reactions.IgM titers of >1:20 are indicative of recent infection with that specific chlamydial agent; however, antichlamydial IgM antibody is very cross reactive and will often demonstrate titers to multiple chlamydial species.
Any IgG titer may indicate past exposure to that particular species. Cross reactive antibody or the presence of nonspecifically stimulated antichlamydial IgG antibody when seen is typically > 1:128. Infection with a particular chlamydial species typically yields antibody titers which are higher than antibody titers to this noninfecting species. IgG titers in recently infected individuals are typically >1:512.
IgA titers may help to identify the infecting Chlamydia species when cross reactive IgG is present. IgA is typically present at low titers during primary Chlamydia infection, but may be elevated in recurrent exposures or in chronic infection.
If attempting to diagnose lymphogranuloma venereum (LGV) infection, request antibodies to chlamydia trachomatis serovars L1, L2 and L3.