Use to establish the diagnosis of Chlamydia trachomatis infection in suspected cases of conjunctivitis, pneumonitis, urethritis, proctitis and cervicitis. Test includes tissue culture technique for the isolation of Chlamydia trachomatis. Identification of inclusions with monoclonal antibody.
Viral transport medium (available from Clinical Microbiology Laboratory)
Obtain the viral transport medium from the Clinical Microbiology laboratory. Wipe the epithelial cell surface to remove any excess mucus. Discard the swab. Using a second swab, vigorously swab the site to be cultured and place the swab directly into the viral transport medium. Place tissue and/or aspirate specimens directly into the viral transport medium. Specimens should be collected early in the acute phase of the infection first 3-5 days after onset.
Specimen not transported in viral transport medium, improper swab used in obtaining specimen, specimen heavily contaminated with bacteria, inappropriate specimen source, specimen collected with a WOODEN swab.
1 mL or swab submitted in viral transport medium.
Specimen should be transported as soon as possible. When transportation is delayed, leave at room temperature.
No Chlamydia isolated
LIMITATIONS OF TEST:
The use of viral transport medium is essential to successful culture.
Tissue culture and direct fluorescent antibody (DFA).
Chlamydia infects the columnar epithelical cells and will not be recovered from squamous epithelial cells or inflammatory cells. In obtaining the specimen, clean the area of inflammatory cells and then attempt to scrape epithelial cells for culturing. Patients in whom Chlamydia trachomatis is isolated are reported to the City of Chicago Board of Health. This is mandated by law.