The test includes a semi-quantitative culture via the Maki method; isolation and identification of microorganisms with greater than 15 colonies, and susceptibility testing, if appropriate. Other isolated organisms (i.e. yeast, mycobacteria, etc.) may be referred for identification and/or susceptibility testing if medically indicated AND a separate culture procedure has NOT yielded the same organism(s).
Daily, 24 hours
Preliminary culture reports available after 24 hours. Culture reports of no growth will be issued after 72 hours. Completion of culture reports may take 3-4 days after receipt of the specimen when multiple isolates are found.
The specific anatomic site of the specimen MUST be specified on the order or the requisition.
Aseptically prepare the culture site. Remove the tip or device without contact with adjacent skin. Contamination with normal flora from skin or other body surfaces MUST be avoided since colonizing bacteria not involved in the infectious process may be introduced onto the sample.
Catheter tip or prosthetic device
Inappropriate specimen container. Urinary catheter tips (i.e. Foley catheters) are unacceptable specimens for culture.
Specimen MUST be transported as soon as possible. When transportation is delayed, refrigerate.
No organisms isolated
LIMITATIONS OF TEST:
Specimens MUST be transported to the laboratory IMMEDIATELY to eliminate desiccation of the specimen.
Semi-quantitative culture via Maki method (roll plate technique).
The presence of 15 or more colonies indicates that the catheter is the possible etiologic source of bacteremia and, perhaps, septic thrombophlebitis. Therefore, the presence of fewer than 15 colonies will be reported as a description of the isolate(s) only. Complete identification and susceptibility testing will not be performed. Identification and susceptibility testing, if appropriate, will be performed when 15 or more colonies are present. Susceptibilities are not routinely performed for coagulase-negative staphylococci, alpha-Streptococcus species, and Corynebacterium species unless isolated from a blood culture obtained during the same day. Gram stains are NOT performed.