The test includes the isolation and identification of microorganisms and susceptibility testing, when appropriate. Other isolated organisms (i.e. anaerobes, yeast, etc.) may be referred for identification and/or susceptibility testing if medically indicated AND a separate culture procedure has NOT yielded the same organism(s).
Daily, 24 hours
The order or requisition MUST state blood for Brucella Culture. Inform the Clinical Microbiology Laboratory of the specific request for Brucella Blood Culture such that the length of incubation of the culture media can be extended. The specific site of the specimen (i.e. arterial, CVP, etc.) MUST be specified on the requisition..
0.1 mL (optimal volume for blood is 10 mL per bottle)
Blood cultures should be transported as soon as possible. When transportation is delayed, leave at room temperature. Do NOT refrigerate. Clients: incubate Bact/Alert bottles if transportation is delayed more than 4 hours.
LIMITATIONS OF TEST:
Blood cultures for Brucella are primarily useful in the early acute phase of the disease. Recovery of Brucella is limited by the relatively low level of bacteremia and the fastidious nature of the organism.
Automation (continuous monitoring)
Serologic testing of acute and convalescent blood specimens for Brucella antibodies may be useful when cultures are negative. The Clinical Microbiology Laboratory MUST be notified when Brucella is suspected so as to optimize the isolation of the organism and to prevent laboratory acquired infections.
Brucella, Blood Culture; Culture for Brucella, Blood