The test includes a direct Gram stain, isolation and identification of microorganisms, and susceptibility testing, if appropriate. Other isolated organisms (i.e. anaerobes, yeast, etc.) may be referred for identification and/or susceptibility testing if medically indicated AND a separate culture procedure has NOT yielded the same organism(s).
Daily, 24 hours
Gram stain: 2 hours after receipt of the specimen in the laboratory. Preliminary culture reports are available after 24 hours. Culture reports of no growth will be issued after 72 hours. Completion of culture reports may take 3-5 days after receipt of the specimen when multiple isolates are found.
All biopsy/tissue specimens will routinely be cultured aerobically and anaerobically unless specifically indicated on the order or requisition that "No anaerobic culture needed." The specific anatomic site of the biopsy MUST be specified on the order or requisition with surface and/or deep or surgical biopsies delineated.
Specimens are to be collected from a site prepared utilizing aseptic technique. Overlying and adjacent areas must be carefully prepared to eliminate surface organisms. Ideally, material is obtained by needle aspiration through intact surface, which has been cleaned with antiseptic, and injected into a sealed, sterile container. Sampling of open lesions is enhanced by deep aspiration using a sterile plastic catheter. If irrigation is necessary, nonbacteriostatic sterile saline may be used. Pulmonary samples may be obtained by transtracheal percutaneous needle aspiration. Optimal isolation of microorganisms from tissue is accomplished by processing as much tissue as possible.
Surgical tissue, biopsy material, etc.
Inappropriate specimen container, insufficient specimen volume, refrigerated specimen, specimen received in a preservative.
0.5-5 mL or a small piece of tissue
Specimen should be transported as soon as possible. When transportation is delayed, leave at room temperature.
LIMITATIONS OF TEST:
A specimen with additional microbiologic procedures will be processed only if the specimen is of adequate quantity for all tests requested. A physician will be contacted to prioritize requested procedures when the specimen is of an inadequate quantity. The portion of the surgical specimen submitted for microbiologic analysis must be separated from the portion submitted to the Pathology Department utilizing sterile technique.