All biopsy specimens will be processed for routine anaerobic culture when an aerobic culture is requested. Test includes culture for anaerobic organisms. Other isolated organisms (i.e. aerobes, yeast, etc.) may be referred for identification and/or susceptibility testing if medically indicated AND a separate culture procedure has NOT yielded the same organism(s).
Daily, 24 hours
Cultures are examined every 48 hours. Preliminary reports are available after 48 hours. Culture reports of no growth will be issued after 5 days. Completion of culture reports may take 7-10 days after receipt of the specimen, depending upon the nature of the microorganisms isolated.
Avoid exposure to atmospheric oxygen. Specimens should be transported as soon as possible. When transportation is delayed, leave at room temperature. Do NOT refrigerate. The specific anatomic site of the biopsy MUST be specified on the requisition with surface and/or deep or surgical biopsies delineated.
The specimen of choice is tissue or an aspirate, NOT a swab. Specimens are to be collected from a site prepared utilizing aseptic technique. Overlying and adjacent areas must be carefully prepared to eliminate surface (normal flora) anaerobes. Ideally, material obtained by needle aspiration through intact surface, which has been cleaned with antiseptic, is put directly into the BBLTM Port-A-Cul Transport System. Sampling of open lesions is enhanced by deep aspiration using a sterile plastic catheter. Curetting of the base of an open lesion is optimal. If irrigation is necessary, nonbacteriostatic sterile saline may be used. Pulmonary samples may be obtained by transtracheal percutaneous needle aspiration. When a syringe is used to obtain the specimen, all air should be expelled after which the sample should be injected into a BBLTM Port-A-Cul Transport System. Swabs should NOT be used due to the small volume of specimen obtainable by this method. Place tissue directly into the transport tube. Secure the lid tightly. Contamination with normal flora from skin or other body surfaces MUST be avoided since colonizing bacteria not involved in the infectious process may be introduced into the sample.
Surgical tissue, biopsy material from normally sterile site appropriately obtained for anaerobic culture.
Inappropriate specimen container, insufficient specimen volume, refrigerated specimen. Specimens from sites which have anaerobic bacteria as normal flora will routinely be rejected.
0.5-10 mL or a small piece of tissue
Avoid exposure to atmospheric oxygen. Specimen should be transported as soon as possible. When transportation is delayed, leave at room temperature. Do NOT refrigerate.
No growth of anaerobic bacteria
LIMITATIONS OF TEST:
A specimen received in anaerobic transport devices is not suitable for routine fungal or mycobacterial cultures. If more than three anaerobic isolates are present, the Bacteroides fragilis group, pigmented gram-negative rods (Prevotella and/or Porphyromonas species), Actinomyces species, and Clostridium perfringens will be the only isolates identified. Anaerobic susceptibility testing is NOT performed routinely. Should susceptibility testing be required, call the Clinical Microbiology laboratory at (312)942-5452.
Conventional culture utilizing media specifically formulated for anaerobic isolation.
The portion of the surgical specimen submitted to the Clinical Microbiology Laboratory should be aseptically separated from the portion submitted to the Pathology Department.