The following are general guidelines for collecting and storing breast milk when using a hospital-grade electric breast pump. If the NICU gives you more specific instructions, follow their recommendations.

Getting ready:

Before you begin to pump:

• Be sure to read the instruction manual for the breast pump and collection kit you are using. (Both items should be manufactured by the same company.)
  
  
• Breast milk is not sterile, but you do not want to introduce "outside" bacteria when getting ready to pump, during the actual pumping session, or when storing milk or transporting it to the NICU.
  
  
  • Always wash and rinse your hands thoroughly before handling any clean pump parts, your breasts, or the milk collection bottles/containers.
    
    
  • Certain pump collection kit parts must be cleaned and sterilized, according to the instruction manual. Most recommend thorough cleaning of these parts after each use and sterilizing parts at least once in 24 hours.
    
    
  • The collection bottles/containers that attach to the pump and are used to collect and store your milk should be sterile. (The NICU may be able to supply the sterile collection containers.)
    
    
• Read the instruction manual first, but you may have to experiment with different techniques and settings on the breast pump before you find ones that work best for you. The following are some general tips:
  
  
  • Some mothers moisten the rim of the breast flange before pumping to create a better seal on the breast.
    
    
  • Some mothers prefer to center the breast flanges on the nipple and areola first and then turn the pump on; others turn the pump on first and then place the flanges over the breasts.
    
    
  • Start the pump at the low/minimal suction setting and gradually move the setting to increase the level of suction. The level usually is set as high as comfort allows. Decrease the suction if it causes discomfort.
    
    
  • Suction cannot be maintained if the seal of the flange on the breast is broken, so check the seal of the flange periodically. Also watch for the rhythmic pull and release of the nipple and areola within the flange.
    
    
  • Expect to pump for a few minutes before you see a steady flow of milk.
    
    
  • Do not fill collection bottles more than 2/3 full to avoid any back flow of milk and to allow for expansion if milk is to be frozen. If you easily fill bottles, have additional collection bottles ready. Stop and change bottles as needed. If your baby takes more than the amount in one bottle at a feeding, you might attach collection bottles that can hold a larger amount to the breast flange.
    
    
  • When you are ready to stop pumping, use a clean finger to press in on your breast just above the pump breast flange. This should break the seal between the flange and the breast tissue. If milk has pooled in a flange, tilt it so that milk can drain into the collection bottle as you remove the flange. Then turn off the breast pump. (Some mothers turn the breast pump off first, and then break the seal between the flange and the breast.)

Storing your breast milk:

• combining milk
  If you pumped both breasts at once and the total amount of milk obtained will fill one bottle no more than 2/3 full, you may combine the contents in one bottle by carefully pouring the milk from one sterile container into the other. Do not combine milk from different pumping sessions when pumping for a high-risk baby.
  
  
• label the collection container(s) immediately
  Labels should include the baby's name, the date, the time of day obtained, and any medications or substances, such as cigarette by-products that you have taken, or been exposed, to since the last pumping session.
  
  
• fresh breast milk
  "Fresh breast milk" contains the most active anti-infective properties. Refrigerated breast milk has fewer anti-infective properties than fresh milk and frozen breast milk has the least.
  
  
  • If using unrefrigerated, fresh breast milk, it should be fed to a baby within an hour of being pumped. Do not leave milk out longer than 30 to 60 minutes when it is to be given to a high-risk baby. This risks contamination - something a high-risk baby does not need.
    
    
  • It is not always possible to give a baby fresh breast milk or you may obtain more than needed for a feeding, so refrigerate any other milk in the labeled collection bottles immediately. The refrigerator should be at a temperature of 32° F to 39° F.
    
    
  • Freeze labeled collection bottles if the milk will not be used within 24 to possibly 48 hours. (The NICU staff will let you know whether they are using 24 hours or 48 hours as a guideline.)
    
    
  • Do not freeze breast milk that has been refrigerated for more than 24 to 48 hours. Although milk has been shown to be safe when refrigerated for several days, experts usually recommend freezing milk sooner when it is to be given to a high-risk baby.
    
    
• frozen breast milk
  
  
  • two to four weeks if the freezer compartment is within the refrigerator. (You must open the refrigerator door to reach the freezer with this model.)
    
    
  • three to four months in a freezer that is part of a refrigerator unit but has a separate door.
    
    
  • six (or more) months in a separate, 0° F "deep" freezer.
    
    
• transporting refrigerated or frozen milk
  • Place it in an insulated bag or cooler with a cool pack. The farther you live from the NICU, the more likely it is that you will have to pad the inside of the cooler with extra cold packs to keep frozen milk from thawing.

Thawing breast milk:

The following are general guidelines for thawing frozen milk:

• The oldest milk should be used first, unless recently expressed milk is recommended.
  
  
• Thaw breast milk by placing the collection container in the refrigerator. It thaws more quickly when held under warm running water or if it is placed in a cup, pot, bowl, or basin of warm water.
  
  
• Do not thaw breast milk at room temperature, in very hot water, or in the microwave. Microwaving can create hot spots. Both microwaving and heating in very hot water may decrease the amount of certain anti-infective properties in the milk.
  
  
• Your milk separates during storage and the cream rises to the top, because breast milk is not homogenized. Gently swirl, or rotate, the collection bottle of milk to mix it together. Avoid vigorous shaking.
  
  
• Do not refreeze milk once it has been thawed. Thawed milk must be used within 24 hours for a baby in the NICU. (It is safe to give milk that has been thawed for 24 to 48 hours after the baby is home.)

Taking care of your breast pump and collection kit:

It is important to maintain the breast pump and collection kit in good working order:

• Read the instruction manual and follow the recommendations for cleaning pump equipment, unless given special guidelines by the NICU.
  
  
• The pieces of the collection kit come apart for individual cleaning.
  
  
• The pump itself and the tubing portion of the kit do not have to be cleaned, and they should remain dry or they will not work appropriately. (These pieces of equipment never come in contact with milk that is to be stored for the baby.)
  
  
• After each use, rinse all parts that come in contact with the breast or milk in cool water first. (A cool rinse removes residual milk without coagulating hard-to-clean protein.) Then thoroughly clean these same parts in hot, soapy water. Rinse in hot water, and air dry between each use.
  
  
• Most manufacturers recommend boiling/sterilizing all parts that come in contact with the breast or milk once a day. (Check the instruction manual.)

Do not use a dishwasher to clean or sterilize the parts that come in contact with the breast or milk unless you have received permission from the NICU and the instruction manual suggests this method as an option.

Pediatric Care at Rush

Rush University Medical Center in Chicago, Illinois, is a leader in caring for children of all ages, from newborns through young adults.

At Rush, physicians from more than 30 specialties address the full range of pediatric diseases and congenital problems, from the common to the complex. Rush pediatricians, pediatric surgeons, nurses and other health professionals provide care that aims to fulfill community needs, empower parents and advance pediatric care.

For more information about the pediatric care at Rush visit the Rush Children’s Hospital home page.

Or phone (888) RUSH-KID or (888) 787-4543.

Looking for Other Health Information?

Visit Discover Rush’s Web Resource page to find articles on health topics and recent health news from Rush University Medical Center in Chicago, Illinois. You will also find many helpful links to other areas of our site.